All-Inclusive Service
RBP-eCLIP
A robust and reproducible framework to map RBP binding sites on RNAs transcriptome-wide
CONTACT US
The RBP-eCLIP Service
Turnaround Time
6-8 Weeks (Project Dependent)
*Requires Validated Antibody
See List of Pre-Validated Antibodies
Starting Material
Cells: 20 M; UV Crosslinked
Tissue: 80 mg; Non-Crosslinked; Flash Frozen
Start your RBP-eCLIP Service Project
Connect With An Eclipsebio RNA Expert
Bioinformatics
Our Comprehensive Data Analysis Package
Eclipsebio’s standard analysis uses human (hg38), mouse (mm10), rat (rn6), or c. elegans (ce11). Contact us for alternate species including viral genomes.
RAW SEQUENCING DATA | FASTQ
Reads directly off sequencer and demultiplexed per sample for data back-up and publication
GENOME ALIGNMENTS | BAM
Reads filtered of repetitive elements, aligned, and PCR deduplicated for additional downstream analysis
COVERAGE TRACKS | BIGWIG
Normalized read coverage on positive and negative strands for visualization in a genome viewer such as IGV
SCORED PEAKS | BED
Genomic regions scored after input normalization as putative binding sites (aka “peaks”) for target exploration. Includes log2 enrichment scores and p-values per peak.
BINDING SITE SUMMARY REPORT | HTML
Report for enriched peaks with interactive tables and plots for publication ready graphics. Includes enriched GO terms, KEGG pathways, motifs, and repetitive element analysis.
