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“My lab had a fantastic experience working with Eclipse Bioinnovations. They made the experiment we sought to do approachable, and the staff was available to help and discuss the project every step of the way.”
“As compared to the other CLIP procedures, the eCLIP kit from Eclipse Bioinnovations includes several steps that improves identification of specific protein-nucleic acid interactions.This kit includes almost all necessary reagents as well as a very detailed protocol with essential explanations of the procedure, suggested stop points and potential trouble for each step of the procedure that makes you well prepared for a relatively long protocol, even if you do not have much experience with CLIP techniques. Kit provides sufficient amount of high quality reagents. Importantly, when testing this kit our control sample produced data that was in very good agreement with previously described protein-nucleic acid interactions.In addition, the highly knowledgeable Eclipse customer support scientists were very helpful and provided necessary assistance in clarification of procedure and interpretation of the results.”
What is eCLIP?
Enhanced CrossLinking and Immunoprecipitation (eCLIP), followed by high-throughput sequencing (eCLIP-seq), is a methodology for identifying RNA transcripts bound in vivo by a protein of interest.Learn more about eCLIP
What proteins can be assayed by eCLIP?
To perform eCLIP for a protein of interest, all that is needed is an antibody that can successfully immunoprecipitate the desired protein. We have a pre-validated list of antibodies for 150 proteins, and can provide validation services for additional or custom antibodies.Learn more about validated eCLIP antibodies
What biological samples
can be assayed with eCLIP?
eCLIP can be performed in a wide variety of biological materials, including immortalized cell lines (e.g. HEK293T, K562, HepG2), stem cell and differentiated populations (e.g. ES and IPSC cells, differentiated neuronal precursor cells or neurons) and tissues or organisms (including mouse and human tissues, C. elegans, and more).
Human & Mouse Tissue
How can I get additional information?
The eCLIP technology was developed by Professor Gene Yeo’s lab at UCSD. Read the eCLIP paper published in Nature Methods to learn more.Read Now