All-Inclusive Service

eRibo Pro

A proprietary ribosome profiling service that streamlines translational insights

The eRibo Pro Service

Turnaround Time
Service: 6-8 Weeks (Project Dependent)

Starting Material
5M Cells
70% – 80% Confluency

Start your eRibo Pro Service Project
Connect With An Eclipsebio RNA Expert

Eclipsebio is sole licensee of the Ribo-seq method covered
by U.S. Patent Numbers 8,486,865, 9,284,547 and 9,677,068.

Bioinformatics

Our Comprehensive Data Analysis Package

Eclipsebio’s standard analysis uses human (hg38). Contact us for alternate species including viral genomes.

EXAMPLE REPORT

RAW SEQUENCING DATA | FASTQ

Reads directly off sequencer and demultiplexed per sample for data back-up and publication

GENOME ALIGNMENTS | BAM

Reads filtered of repetitive elements, aligned, and PCR deduplicated for additional downstream analysis

COVERAGE TRACKS | BIGWIG

Normalized read coverage on positive and negative strands for visualization in a genome viewer such as IGV

DIFFERENTIAL EXPRESSION & RIBOSOME OCCUPANCY | TSV

Results and statistics table for differential gene count analysis using DESeq2 for the RPF and RNA-Seq libraries, and for the calculated ribosome occupancy. Includes log2 fold changes and p-values

eRibo SUMMARY REPORT | HTML

Report comparing libraries from ribosome associated (RPF) and RNA-Seq for a given sample and for samples from two different conditions with interactive tables and plots for publication ready graphics. Includes QC metrics, visualization of ontology enrichment, and differential genes