eBlogs
A DE analysis is a statistical procedure that identifies differentially up or downregulated genes between two or more conditions or samples. It involves comparing the expression levels of each gene in one group of samples (e.g., disease samples) to the expression levels in another (e.g., healthy samples) to identify genes that have changed across conditions.... [READ MORE]
eBlogs
The goal of RBP-eCLIP is to identify where an RNA-binding protein (RBP) is binding; these regions are often called peaks due to their mountain-like appearance on a genome browser. After peaks have been called, it is important to determine what genes and gene features are associated with those sites.... [READ MORE]
eBlogs
Forward, reverse, sense, antisense, first strand, second strand, unstranded. Different methods for sequencing RNA-Seq data can lead to differently stranded libraries all with different names. This can make it challenging to figure out how different kits compare to one another or what parameters to use with different software tools to make sure you are doing an analysis correctly.... [READ MORE]
