The eCLIP kit is a simplified and robust library preparation method to identify your RNA-binding proteins targets.
Eliminate time-consuming prep work by ordering one kit with 30+ qualified reagents. Start immediately and generate eCLIP libraries in only 4 days.
Published method and protocol improvements increase workflow efficiency and identify true binding sites with single nucleotide resolution
Kits include validated protocol and qualified reagents to ensure reproducibility for better results.
You can also add data analysis to your eCLIP kit library preparation kit
Our eCLIP library preparation kit is an optimized 4-day protocol based on Van Nostrand, Yeo et al. Nature Methods 2016 paper.
Based on Van Nostrand, Yeo et al. Nature Methods 2016 paper
Why choose the eCLIP kit?
Each kit includes:
Simplified and robust identification of your RNA-binding protein targets in one week
eCLIP Kit Protocol (v1.01R)
What do I need to begin an eCLIP experiment?
Each eCLIP experiment uses an antibody to immunoprecipitate the RNA binding protein of interest from a biological sample (cells, tissues, or other biospecimen).
- Pre-validated antibodies are available for 150 RNA binding proteins; alternatively, we recommend antibody validation be performed prior to eCLIP using the eCLIP immunoprecipitation validation kit.
- Biosamples should be UV cross-linked according to protocols available for Suspension Cells and Adherent Cells.
What sequencing format is eCLIP designed for?
The eCLIP kit generates libraries suitable for standard Illumina 50-75nt single-end sequencing. Multiple libraries can be pooled together and demultiplexed using standard Illumina techniques and software.
How many reads should be sequenced per eCLIP library?
Eclipse BioInnovations recommends 20 million sequenced reads for publication purposes. 5-10 million reads is typically suitable for quality assessment.
How do I analyze an eCLIP experiment?
Standard analysis of eCLIP requires read adapter trimming, mapping to the appropriate genome, peak identification, and comparison against a paired input, knockout, or other suitable control sample. An example eCLIP analysis pipeline has been described by the ENCODE consortium here.
Other Tools & Resources
UV Crosslinking Protocols