MicroRNA target identification with chimeric eCLIP

 

microRNAs regulate gene expression through decreasing RNA stability and inhibiting translation

microRNAs are short regulatory RNAs that repress gene expression through binding of complementary sequences in mRNA 3′ Untranslated Regions

Modification of eCLIP enables ligation of microRNAs with mRNA targets to create chimeric fragments that unambiguously map individual microRNA binding sites with high resolution

Chimeric eCLIP directly maps individual microRNA targets

Standard Ago2 eCLIP identifies microRNA landing sites

Whole-transcriptome chimeric eCLIP maps targets of all microRNAs

miR-specific library preparation options enable deep profiling of an individual microRNA of interest

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microRNA targets can be identified with high specificity through Ago2 Chimeric eCLIP.

Contact us for additional information

Please contact us to receive a quote for custom services or to ask any questions you may have. A brief description of your desired experiment (number of samples, custom versus commercial antibody) will enable us to better respond to your queries.